9/18/2023 0 Comments Gapdh western blot kdaReactome: R-HSA-70171 Reactome: R-HSA-70263ĬytoplasmCytosolNucleusPerinuclear RegionMembraneCytoskeletonTranslocates To The Nucleus Following S-Nitrosylation And Interaction With Siah1Which Contains A Nuclear Localization SignalPostnuclear And Perinuclear RegionsĪnti-Glyceraldehyde-3-Phosphate Dehydrogenase antibodyAnti-Gapdh antibodyAnti-Peptidyl-Cysteine S-Nitrosylase Gapdh antibodyAnti-GAPDH antibodyAnti-GAPD antibodyAnti-CDABP0047 antibodyAnti-OK antibodyAnti-SW-cl. Glyceraldehyde-3-Phosphate DehydrogenaseGapdhPeptidyl-Cysteine S-Nitrosylase Gapdh Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. (B) The abundance of CD73 protein was analyzed by Western blotting. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Bars represent mean SEM of CD73-mRNA abundance relative to GAPDH, determined in n. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Modulates the organization and assembly of the cytoskeleton. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. This Anti-GAPDH Antibody product is a new_product replacing p/n 600-401-A33S.Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control marker. Expect a band at ~36 kDa in size corresponding to GAPDH by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Anti-GAPDH Antibody has been tested for use in ELISA and western blotting. Anti-GAPDH antibody is ideal for investigators involved in apoptosis, cancer, DNA damage and repair and neuroscience. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel. A loading control antibody is critical for the correct interpretation of your western blot. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control antibody in immunoblots. GAPDH loading control antibody is ideal for Western Blotting, ELISA, IHC and IF Microscopy.
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